Indian Association of Leprologists


Indian Association
of Leprologists

Indian Association of Leprologists

president@ial-leprosy.org
secretary@ial-leprosy.org

Publication


31st BIENNIAL CONFERENCE 2021 INDIAN ASSOCIATION OF LEPROLOGISTS (IAL) - Abstracts

Indian Association Of Leprologists





IAL Textbook Of Leprosy (Indian Association Of Leprologists)

Indian Association Of Leprologists





IAL HANDBOOK OF LEPROSY 1st Edition

Indian Association Of Leprologists





INDIAN JOURNAL OF LEPROSY

Indian Association Of Leprologists





Lepr Rev (2006) 77, 366370

Isolation of Mycobacterium leprae from untreated borderline tuberculoid, mid-borderline and indeterminate cases using the mouse foot pad technique a study of 209 cases

A. V. WAKADE & V. P. SHETTY
The Foundation for Medical Research, 84- A R.G. Thadani Marg,
Worli, Mumbai 400 018, India

Accepted for publication 29 August 2006

Summary Using the mouse foot pad (MFP) system, isolation of Mycobacterium leprae was attempted in 209 skin biopsies obtained from 114 borderline tuberculoid (BT), 62 mid borderline (BB) and 33 indeterminate (I) untreated cases. Unequivocal growth in the foot pads of mice was seen in 100 (478%) cases. Of these 100 cases that showed growth in the mouse foot pad system, in 20 cases acid fast bacilli (AFB) were detected in small numbers (1 ) in either smear or homogenate. The remaining 80 (42%) cases were negative for AFB in both smear and homogenate. The occurrence of viable bacilli and percentage take at 12 months was highest in BB (76 and 86%) followed by BT (38 and 75%) and I (30% and 52%) cases. In most of the BT (65%) and I (60%) cases, the first peak was seen only at 12 months. These results confirm that viable bacilli can be isolated and expanded from a good proportion of negative BT-BB cases using immunocompetent Swiss White mice.